The animal support and mounting system is a plastic bed fixed on a plastic stand with adjustable support columns on which the perfusion chamber is placed. The perfusion chamber is mounted using M4 bolts, which securely connect the perfusion chamber, support columns and plastic bed altogether. The support and mounting system is a block solution, where each element can be replaced/added at the user’s request. For example, a stand for a plastic bed can be made either in a non-adjustable version or with a ball joint to allow regulation of the relative position of the plastic bed in space.

Superfusion chamber
The superperfusion chamber is both a support for the animal and a supporting structure for attachment to a stereotactic apparatus or other mounting system. The superperfusion chamber is a monolithic product and has the following structure: the body of the perfusion chamber contains three inlet channels, two open chambers: a preliminary chamber and a main chamber, connected by an inlet spiral chute, as well as an opening for the exit of the perfusion solution, connected to the main chamber by an outlet spiral chute. Three input channels are used to inject the perfusion solution, place the reference electrode, and place the perfusion solution temperature sensor, respectively. The input channels are made in the form of through holes. The presence of a preliminary chamber allows you to protect the main chamber from air bubbles and water hammer, which are possible due to artifacts in flow-through perfusion systems. The size of the perfusion chamber can be scaled to the size of any animal being studied.